Affordable Access

Lipid Composition of Outer Leaflet of Chloroplast Outer Envelope Determines Topology of OEP7

Authors
Publisher
The American Society for Cell Biology
Publication Date
Source
PMC
Keywords
  • Article
Disciplines
  • Biology
  • Mathematics

Abstract

Fig. 4. Insertion of OEP7 and variants into liposomes. [35S]Methionine-labeled OEP7, OEP7-LM1, OEP7-LM2, and OEP7-LM3 (A and D) as well as [3H]leucine-labeled OEP7 and OEP7-LM1 (B and D) were incubated with liposomes composed from purified single lipids (A and B) or lipids purified from spinach outer envelope (D). Schleiff E et al. Mol. Biol. Cell 2001;12:4090-4102 Copyright © 2001 The American Society for Cell Biology Insertion of OEP7 and variants into liposomes. [35S]Methionine-labeled OEP7, OEP7-LM1, OEP7-LM2, and OEP7-LM3 (A and D) as well as [3H]leucine-labeled OEP7 and OEP7-LM1 (B and D) were incubated with liposomes composed from purified single lipids (A and B) or lipids purified from spinach outer envelope (D). In A, lane 1 represents 10% translation product (TP), and lane 2 binding and insertion into liposomes before and lane 3 after competition with N-liposomes. Lane 4 represents liposomes after thermolysin (Thr) treatment. In B and D, lane 1 represents 10% TP, and lane 2 binding and insertion into liposomes after competition with N-liposomes. Lane 3 represents liposomes after Thr treatment. In C, the topology of OEP7 and mutants within the liposome bilayer is shown. The position of the radioactive label is indicated. PF, proteolytic fragment; wt, full-length protein).

There are no comments yet on this publication. Be the first to share your thoughts.