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Variation in gene expression profile with aging of Pinus radiata D. Don

BMC Proceedings
Springer (Biomed Central Ltd.)
Publication Date
DOI: 10.1186/1753-6561-5-s7-p62
  • Poster Presentation
  • Biology


Variation in gene expression profile with aging of Pinus radiata D. Don POSTER PRESENTATION Open Access Variation in gene expression profile with aging of Pinus radiata D. Don Carolina Alvarez1*, Luis Valledor2, Rodrigo Hasbún3, Manuel Sanchez-Olate1, Darcy Ríos1 From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery Arraial d’Ajuda, Bahia, Brazil. 26 June - 2 July 2011 Phase change in higher plants, from juvenile to mature, affects the reproductive competence, morphology and growth rate, as well as the regenerative potential of tis- sue explants. However, in conifers these changes can be achieved before the onset of flowering and at the begin- ning of the seed production. Early maturational changes in conifers are very obvious, severe and overall irreversi- ble [1], and are characterized by lost of morphogenetic capacity. This may affect clonal multiplication programs and genetic manipulation, due mainly to the decline in adventitious rooting capacity which is the most marked maturation and aging effect. Despite the serious effects of these processes, little is known about their basic regu- lation in forest clonal propagation programs. The latest researches regarding this subject are related to auxins changes during maturation [2], epigenetic variations during phase-change, like DNA methylation [3], and proteomic changes during needle maturation [4]. This work has been performed to gain deeper insight into the genetic mechanisms that regulates the loss of morpho- genetic capacity with the aging process in P. radiata. P. radiata ortets of different age were brought from La Posada nursery at BioBio region, Chile. Needles were collected in August, frozen in liquid nitrogen and stored at -80°C until its RNA extraction. RNA was isolated from 100 mg of frozen tissue according to Chang [5], the RNA extracts were purified with RNeasy Clean up and treated with RNase-free DNase (both from QIA- GEN). Gene expression analysis was done by a reverse dot blot

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