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The prostaglandin transporter PGT transports PGH2

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
395
Issue
2
Identifiers
DOI: 10.1016/j.bbrc.2010.03.108
Keywords
  • Prostaglandin H2
  • Prostaglandin Transporter
  • Kinetics
Disciplines
  • Biology

Abstract

Abstract Prostaglandin H 2 not only serves as the common precursor of all other PGs, but also directly triggers signals (e.g. platelet aggregation), depending on its location and translocation. The prostaglandin carrier PGT mediates the transport of several prostanoids, such as PGE 2, and PGF 2α. Here we used PGT in the plasma membrane as a model system to test the hypothesis that PGT also transports PGH 2. Using wild-type and PGT-expressing MDCK cells, we show that PGH 2 uptake is mediated both by simple diffusion and by PGT. The PGH 2 influx permeability coefficient for diffusion is (5.66 ± 0.63) × 10 −6 cm/s. The kinetic parameters of PGH 2 transport by PGT are K m = 376 ± 34 nM and V max = 210.2 ± 11.4 fmol/mg protein/s. PGH 2 transport by PGT can be inhibited by excess PGE 2 or by a PGT inhibitor. We conclude that PGT may play a role in transporting PGH 2 across cellular membranes.

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