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High-performance size-exclusion chromatography: A buffer for the reliable determination of molecular weights of proteins

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
121
Issue
2
Identifiers
DOI: 10.1016/0003-2697(82)90496-1
Disciplines
  • Biology

Abstract

Abstract A high-performance size-exclusion chromatographic system is described that minimizes the ionic and hydrophobic interactions of proteins with the stationary phase. The system can be used to determine reliably the molecular weight of proteins between M r 10,000 and 70,000. A further application would be to separate proteins on a preparative (milligram) scale. Conditions critical for optimal resolution are discussed.

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