Affordable Access

A simple and sensitive method for determining the strand orientation of single-stranded viral genomes

Authors
Journal
Journal of Virological Methods
0166-0934
Publisher
Elsevier
Volume
185
Issue
1
Identifiers
DOI: 10.1016/j.jviromet.2012.05.017
Keywords
  • Virus Genome
  • Single-Stranded Dna
  • Strand Orientation
  • Sispa

Abstract

Abstract Determining the nature of the viral genome is one of the first steps in characterization of any new virus. However, in the case of viruses with a single-stranded genome, it is not always simple to identify its orientation. In this study, a rapid, sensitive and simple PCR-based method is described to identify the strand orientation of single-stranded viral genomes. This method has been tested on the single-stranded DNA viruses, M13 and phiX174.

There are no comments yet on this publication. Be the first to share your thoughts.