Affordable Access

Publisher Website

Stability indicating RP-LC determination of sildenafil citrate (Viagra) in pure form and in pharmaceutical samples

Authors
Journal
Journal of Pharmaceutical and Biomedical Analysis
0731-7085
Publisher
Elsevier
Publication Date
Volume
29
Issue
4
Identifiers
DOI: 10.1016/s0731-7085(02)00123-1
Keywords
  • Rp-Hplc
  • Sildenafil Citrate
  • Piroxicam
  • Internal Standard
  • Pharmaceutical Formulations
Disciplines
  • Pharmacology

Abstract

Abstract A simple, precise, sensitive and stability-indicating reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitation of sildenafil citrate (SC) in pure form and its pharmaceutical formulations. Method employs water and acetonitrile (48:52 v/v) as mobile phase with flow rate of 1 ml min −1, LiChrospher C18-5 μm (25×0.46 cm) column and UV detection set at 245 nm. The internal standard method using piroxicam (PX) as the internal standard is used. The linear dynamic range of SC was found to be 0.05–7.5 μg ml −1. The proposed method is successfully employed for the determination of SC in the tablets. The excipients present in the formulations do not interfere with the assay procedure. Analytical parameters were calculated and full statistical evaluation is included.

There are no comments yet on this publication. Be the first to share your thoughts.