Affordable Access

deepdyve-link
Publisher Website

Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation.

Authors
  • Gantumur, Enkhtuul1
  • Nakahata, Masaki1
  • Kojima, Masaru1
  • Sakai, Shinji1
  • 1 Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan. , (Japan)
Type
Published Article
Journal
International journal of bioprinting
Publication Date
Jan 01, 2020
Volume
6
Issue
1
Pages
250–250
Identifiers
DOI: 10.18063/ijb.v6i1.250
PMID: 32596552
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We report an extrusion-based bioprinting approach, in which stabilization of extruded bioink is achieved through horseradish peroxidase (HRP)-catalyzed cross-linking consuming hydrogen peroxide (H2O2) supplied from HRP and glucose. The bioinks containing living cells, HRP, glucose, alginate possessing phenolic hydroxyl (Ph) groups, and cellulose nanofiber were extruded to fabricate 3D hydrogel constructs. Lattice- and human nose-shaped 3D constructs were successfully printed and showed good stability in cell culture medium for over a week. Mouse 10T1/2 fibroblasts enclosed in the printed constructs remained viable after 7 days of culture. It was also able to switch a non-cell-adhesive surface of the printed construct to cell-adhesive surface for culturing cells on it through a subsequent cross-linking of gelatin possessing Ph moieties. These results demonstrate the possibility of utilizing the presented cross-linking method for 3D bioprinting. Copyright © 2020, Whioce Publishing Pte. Ltd.

Report this publication

Statistics

Seen <100 times