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Extracts of Cynomorium songaricum protect SK-N-SH human neuroblastoma cells against staurosporine-induced apoptosis potentially through their radical scavenging activity.

Authors
  • Lu, Yi
  • Wang, Qingguo
  • Melzig, Matthias F
  • Jenett-Siems, Kristina
Type
Published Article
Journal
Phytotherapy Research
Publisher
Wiley (John Wiley & Sons)
Publication Date
Feb 01, 2009
Volume
23
Issue
2
Pages
257–261
Identifiers
DOI: 10.1002/ptr.2605
PMID: 18844290
Source
Medline
License
Unknown

Abstract

In traditional Chinese medicine a number of herbs are used to alleviate the symptoms of aging, among them the stems of Cynomorium songaricum, Cynomoriaceae. This study evaluated the protective effect of different extracts of C. songaricum on staurosporine-induced apoptotic cell death in SK-N-SH neuroblastoma cells. Staurosporine (100 nm) reduced cell viability to about 55%. The ethyl acetate fraction of C. songaricum significantly attenuated staurosporine-induced cell death at concentrations of 100 and 10 microg/mL. On the other hand, the dichloromethane as well as water fractions showed no protective effects. In order to further analyse the protective mode of action, the superoxide anion scavenging activity of two extracts was evaluated in a xanthine/xanthine oxidase system. In this system, the EtOAc extract showed a good scavenging activity (IC(50) value 2.9 microg/mL) without inhibition of xanthine oxidase. In conclusion, the results prove the neuroprotective activity of C. songaricum extracts in vitro, thus supporting its traditional use.

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