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Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5'-hydroxypiroxicam in human plasma and urine.

Authors
  • Avgerinos, A
  • Axarlis, S
  • Dragatsis, J
  • Karidas, T
  • Malamataris, S
Type
Published Article
Journal
Journal of chromatography. B, Biomedical applications
Publication Date
Nov 03, 1995
Volume
673
Issue
1
Pages
142–146
Identifiers
PMID: 8925068
Source
Medline
License
Unknown

Abstract

A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection, at 330 nm, was developed for the simultaneous determination of piroxicam and its major metabolite, 5'-hydroxypiroxicam, in human plasma and urine. Acidified plasma and alkali-treated urine samples are used and naproxen is added as internal standard. The separation is performed at 40 degrees C on a C18 Spherisorb column with acetonitrile--0.1 M sodium acetate (33:67, v/v, pH 3.3) as mobile phase. The retention time is 2.2 min for 5'-hydroxypiroxicam, 2.6 min for piroxicam and 3.2 min for naproxen. The detection limit is 0.05 micrograms/ml using a 100-microliters loop.

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