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Extraction and detection of Mycobacterium leprae DNA from ZNCF-stained skin smear slides for better identification of negative skin smears.

Authors
  • Kamble, R R1
  • Shinde, V S
  • Madhale, S P
  • Kamble, A A
  • Ravikumar, B P
  • Jadhav, R S
  • 1 Stanley Browne Laboratory, TLM Community Hospital, Nand Nagari Delhi - 110 093, India. , (India)
Type
Published Article
Journal
Indian journal of medical microbiology
Publication Date
Jan 01, 2010
Volume
28
Issue
1
Pages
57–59
Identifiers
DOI: 10.4103/0255-0857.58732
PMID: 20061767
Source
Medline
License
Unknown

Abstract

Abstract Identification of Mycobacterium leprae, which causes leprosy, is done by Ziehl Neelsen Carbol Fuchsin (ZNCF) stained slit skin smear microscopy that aids in the diagnosis and quantification of approximate bacterial load carried by the patient. We attempted M. leprae DNA extraction from 46 stained slit skin smear negative slides, using Proteinase K and SDS lysis, followed by ethanol precipitation. M. leprae specific primers (16SrRNA) were used for PCR-based amplification of DNA. We could detect M. leprae DNA in 15 (32.6%) samples. The method can be useful in the diagnosis of apparently slit skin smear negative leprosy cases.

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