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Extracting three-dimensional orientation and tractography of myofibers using optical coherence tomography.

Authors
Type
Published Article
Journal
Biomedical Optics Express
2156-7085
Publisher
The Optical Society
Publication Date
Volume
4
Issue
10
Pages
2150–2165
Identifiers
DOI: 10.1364/BOE.4.002150
PMID: 24156071
Source
Medline
Keywords
License
Unknown

Abstract

Abnormal changes in orientation of myofibers are associated with various cardiac diseases such as arrhythmia, irregular contraction, and cardiomyopathy. To extract fiber information, we present a method of quantifying fiber orientation and reconstructing three-dimensional tractography of myofibers using optical coherence tomography (OCT). A gradient based algorithm was developed to quantify fiber orientation in three dimensions and particle filtering technique was employed to track myofibers. Prior to image processing, three-dimensional image data set were acquired from all cardiac chambers and ventricular septum of swine hearts using OCT system without optical clearing. The algorithm was validated through rotation test and comparison with manual measurements. The experimental results demonstrate that we are able to visualize three-dimensional fiber tractography in myocardium tissues.

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