Affordable Access

Extracting DNA twist rigidity from experimental supercoiling data.

Authors
Type
Published Article
Journal
Physical review letters
Publication Date
Volume
93
Issue
19
Pages
198107–198107
Identifiers
PMID: 15600890
Source
Medline
License
Unknown

Abstract

We use an elastic rod model with contact to study the extension versus rotation diagrams of single supercoiled DNA molecules. We reproduce quantitatively the supercoiling response of overtwisted DNA and, using experimental data, we obtain an estimate of the effective supercoiling radius and of the twist rigidity of B-DNA. We find that the twist rigidity of DNA seems to vary widely with the nature and concentration of the salt buffer in which it is immersed.

Statistics

Seen <100 times