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Extracellular matrix mimetic peptide scaffolds for neural stem cell culture and differentiation.

Authors
  • Mammadov, Busra
  • Guler, Mustafa O
  • Tekinay, Ayse B
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
2014
Volume
1202
Pages
131–148
Identifiers
DOI: 10.1007/7651_2013_35
PMID: 24519002
Source
Medline
License
Unknown

Abstract

Self-assembled peptide nanofibers form three-dimensional networks that are quite similar to fibrous extracellular matrix (ECM) in their physical structure. By incorporating short peptide sequences derived from ECM proteins, these nanofibers provide bioactive platforms for cell culture studies. This protocol provides information about preparation and characterization of self-assembled peptide nanofiber scaffolds, culturing of neural stem cells (NSCs) on these scaffolds, and analysis of cell behavior. As cell behavior analyses, viability and proliferation of NSCs as well as investigation of differentiation by immunocytochemistry, qRT-PCR, western blot, and morphological analysis on ECM mimetic peptide nanofiber scaffolds are described.

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