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Extension of the lifespan of cultured normal human diploid cells by vitamin E.

Authors
  • Packer, L
  • Smith, J R
Type
Published Article
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Publication Date
Dec 01, 1974
Volume
71
Issue
12
Pages
4763–4767
Identifiers
PMID: 4531015
Source
Medline
License
Unknown

Abstract

Inclusion of vitamin E (DL-alpha-tocopherol) in the culture medium for human diploid cells greatly prolongs their in vitro lifespan. The addition of 100 mug of DL-alpha-tocopherol per ml of medium has allowed us to culture WI-38 cells for more than 100 population doublings to date. (These cells normally have an in vitro lifespan of 50 +/- 10 population doublings.) Cells at the 100th population doubling have a normal diploid karyotype, appear to behave in all other respects like young WI-38 cells, and are still actively dividing. We interpret this result as support for the free radical theory of aging.

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