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Extension of the lifespan of cultured normal human diploid cells by vitamin E.

Authors
  • Packer, L
  • Smith, J R
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Dec 01, 1974
Volume
71
Issue
12
Pages
4763–4767
Identifiers
PMID: 4531015
Source
Medline
License
Unknown

Abstract

Inclusion of vitamin E (DL-alpha-tocopherol) in the culture medium for human diploid cells greatly prolongs their in vitro lifespan. The addition of 100 mug of DL-alpha-tocopherol per ml of medium has allowed us to culture WI-38 cells for more than 100 population doublings to date. (These cells normally have an in vitro lifespan of 50 +/- 10 population doublings.) Cells at the 100th population doubling have a normal diploid karyotype, appear to behave in all other respects like young WI-38 cells, and are still actively dividing. We interpret this result as support for the free radical theory of aging.

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