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ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L.

Authors
  • Spinedi, Nahuel1
  • Storb, Romina1
  • Aranda, Elisabet2
  • Romani, Facundo3, 4
  • Svriz, Maya5
  • Varela, Santiago A6
  • Moreno, Javier E3
  • Fracchia, Sebastian7
  • Cabrera, Juan1
  • Batista-García, Ramón Alberto8
  • Ponce de León, Inés9
  • Scervino, J Martín1
  • 1 Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA), CONICET-UNCo, SC Bariloche, Río Negro 8400, Argentina. , (Argentina)
  • 2 Institute of Water Research, University of Granada, Ramón y Cajal, 4, Bldg. Fray Luís, 18071 Granada, Spain. , (Spain)
  • 3 Instituto de Agrobiotecnología del Litoral, UNL-Conicet, Facultad de Bioquímica y Ciencias Biológicas, Santa Fe 3000, Argentina. , (Argentina)
  • 4 Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
  • 5 Instituto de Investigaciones en Recursos Naturales, Agroecología y Desarrollo Rural (IRNAD), Universidad Nacional de Río Negro, CONICET, SC Bariloche, Río Negro 8400, Argentina. , (Argentina)
  • 6 Grupo de Ecología Forestal, Instituto Nacional de Tecnología Agropecuaria (INTA) EEA Bariloche, CC 277, Bariloche 8400, Argentina. , (Argentina)
  • 7 Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja (CRILAR), UNLAR, SEGEMAR, UNCa, CONICET, Entre Ríos y Mendoza, 530 Anillaco, La Rioja 5300, Argentina. , (Argentina)
  • 8 Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Ave Universidad 1001, Col. Chamilpa, CP 62209 Cuernavaca, Morelos, Mexico. , (Mexico)
  • 9 Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, CP 11600 Montevideo, Uruguay. , (Uruguay)
Type
Published Article
Journal
Plants
Publisher
MDPI AG
Publication Date
Jul 19, 2021
Volume
10
Issue
7
Identifiers
DOI: 10.3390/plants10071478
PMID: 34371683
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Marchantia polymorpha L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of M. polymorpha to high doses of anthracene analysing the antioxidant enzymes and their relationship with the photosynthetic processes, as well as their transcriptomic response. We found an anthracene dose-dependent response reducing plant biomass and associated to an alteration of the ultrastructure of a 23.6% of chloroplasts. Despite a reduction in total thallus-chlorophyll of 31.6% of Chl a and 38.4% of Chl b, this was not accompanied by a significant change in the net photosynthesis rate and maximum quantum efficiency (Fv/Fm). However, we found an increase in the activity of main ROS-detoxifying enzymes of 34.09% of peroxidase and 692% of ascorbate peroxidase, supported at transcriptional level with the upregulation of ROS-related detoxifying responses. Finally, we found that M. polymorpha tolerated anthracene-stress under the lowest concentration used and can suffer physiological alterations under higher concentrations tested related to the accumulation of anthracene within plant tissues. Our results show that M. polymorpha under PAH stress condition activated two complementary physiological responses including the activation of antioxidant mechanisms and the accumulation of the pollutant within plant tissues to mitigate the damage to the photosynthetic apparatus.

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