Affordable Access

Access to the full text

Expression of Wnt and Notch signaling pathways in inflammatory bowel disease treated with mesenchymal stem cell transplantation: evaluation in a rat model

Authors
  • Xing, Yanfen1, 2
  • Chen, Xiaojie1, 2
  • Cao, Yanwen2, 3
  • Huang, Jianyun1, 2
  • Xie, Xuhong1, 2
  • Wei, Yaming1, 2
  • 1 Guangzhou Medical University, Department of Blood Transfusion, Guangzhou First People’s Hospital, No. 1. Panfu Road, Guangzhou, Guangdong Province, 510180, China , Guangzhou (China)
  • 2 Guangzhou First People’s Hospital, Guangzhou Medical University, Guangdong Key laboratory of Clinical Molecular Medicine and Diagnostics, No. 1. Panfu Road, Guangzhou, Guangdong Province, 510180, China , Guangzhou (China)
  • 3 Guangzhou First People’s Hospital, Guangzhou Medical University, Department of Gastroenterology, No. 1. Panfu Road, Guangzhou, Guangdong Province, 510180, China , Guangzhou (China)
Type
Published Article
Journal
Stem Cell Research & Therapy
Publisher
Springer Nature America, Inc
Publication Date
May 22, 2015
Volume
6
Issue
1
Identifiers
DOI: 10.1186/s13287-015-0092-3
Source
Springer Nature
Keywords
License
Yellow

Abstract

IntroductionThe purpose of this study was to investigate the expression of Wnt and Notch signaling pathway-related genes in inflammatory bowel disease (IBD) treated with mesenchymal stem cell transplantation (MSCT).MethodsTNBS (2,4,6-trinitrobenzene sulfonic acid) was used to establish IBD in a rat model. Mesenchymal stem cells (MSCs) were transplanted via tail vein transfusion. Saline water was used in a control group. The expression of Wnt and Notch main signaling molecules was screened by gene chips and verified by quantitative reverse transcription-polymerase chain reaction in the IBD rat model on day 14 and day 28 after transplantation.ResultsThe IBD rat models were successfully established and MSCs were transplanted into those models. Genome-wide expression profile chips identified a total of 388 differentially expressive genes, of which 191 were upregulated and 197 were downregulated in the MSC-transplanted group in comparison with the IBD control group. Real-time quantitative polymerase chain reaction results showed that the level of Olfm4 mRNA expression in the IBD group (2.54±0.20) was significantly increased compared with the MSCT group (1.39±0.54) and the normal group (1.62±0.25) (P <0.05). The Wnt3a mRNA was more highly expressed in IBD rats (2.92±0.94) and decreased in MSCT rats (0.17±0.63, P <0.05). The expression of GSK-3β mRNA was decreased in the setting of inflammation (0.65±0.04 versus 1.00±0.01 in normal group, P <0.05) but returned to normal levels after MSCT (0.81±0.17). The expression of β-catenin was observed to increase in IBD tissues (1.76±0.44) compared with normal tissues (1.00±0.01, P <0.05), but no difference was found in the MSCT group (1.12±0.36). Wnt11 declined at 14 days and returned to normal levels at 28 days in the IBD group; in comparison, a significantly lower expression was found in MSCT rats. There were no differences in the expression of Fzd3, c-myc, TCF4, and Wnt5a in inflammation, but all of those genes declined after MSCT treatment.ConclusionsThe canonical Wnt and Notch signaling pathways are activated in IBD and may be suppressed by stem cell transplantation to differentiate into intestinal epithelium after MSCT. Moreover, the non-canonical Wnt signaling may be inhibited by canonical Wnt signaling in the setting of inflammation and may also be suppressed by MSCT.

Report this publication

Statistics

Seen <100 times