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Expression of two ecdysteroid-regulated genes, Broad-Complex and E75, in the brain and ovary of the honeybee (Apis mellifera L.).

Authors
  • Paul, Rajib Kumar
  • Takeuchi, Hideaki
  • Kubo, Takeo
Type
Published Article
Journal
ZOOLOGICAL SCIENCE
Publisher
BioOne (Zoological Society of Japan)
Publication Date
Dec 01, 2006
Volume
23
Issue
12
Pages
1085–1092
Identifiers
PMID: 17261922
Source
Medline
License
Unknown

Abstract

We previously demonstrated that two ecdysteroid-regulated genes, Mblk-1/E93 and E74, are expressed selectively in Kenyon cell subtypes in the mushroom bodies of the honeybee (Apis mellifera L.) brain. To further examine the possible involvement of ecdysteroid-regulated genes in brain function as well as in oogenesis in the honeybee, we isolated cDNAs for two other ecdysteroid-regulated genes, Broad-Complex (BR-C) and E75, and analyzed their expression in the worker brain as well as in the queen abdomen. In situ hybridization revealed that BR-C, like Mblk-1/ E93, is expressed selectively in the large-type Kenyon cells of the mushroom bodies in the worker brain, whereas E75 is expressed in all mushroom body neuron subtypes, suggesting a difference in the mode of response to ecdysteroid among Kenyon cell subtypes. In the queen ovary, both BR-C and E75 are expressed preferentially in the follicle cells that surround egg cells at the late stage, suggesting their role in oogenesis. These results suggest that BR-C and E75 are involved in the regulation of brain function as well as in reproductive physiology in the adult honeybee.

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