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Expression of the three influenza virus polymerase proteins in a single cell allows growth complementation of viral mutants.

Authors
  • Krystal, M
  • Li, R
  • Lyles, D
  • Pavlakis, G
  • Palese, P
Type
Published Article
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Publication Date
March 1986
Volume
83
Issue
8
Pages
2709–2713
Identifiers
PMID: 3010315
Source
Medline
License
Unknown

Abstract

Transformed cell lines derived from murine C127 cells were constructed that express the influenza virus RNA-dependent RNA polymerase proteins (PA, PB1, and PB2). Cell lines that express only one or all three of the proteins were tested for their ability to complement temperature-sensitive viral mutants incubated at the nonpermissive temperature. Two cell lines were isolated that express all three polymerase genes and complement the growth of PB2 temperature-sensitive mutants at the nonpermissive temperature. One of these lines also complemented PA temperature-sensitive mutants. The viral titers obtained in these two cell lines were 12-fold to 1000-fold higher than the viral titers obtained upon growth of the corresponding temperature-sensitive mutant in C127 cells at the nonpermissive temperature.

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