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Expression, purification and crystallization of two endonuclease III enzymes from Deinococcus radiodurans.

Authors
  • Sarre, Aili
  • Ökvist, Mats
  • Klar, Tobias
  • Moe, Elin
  • Timmins, Joanna
Type
Published Article
Journal
Acta crystallographica. Section F, Structural biology communications
Publication Date
Dec 01, 2014
Volume
70
Issue
Pt 12
Pages
1688–1692
Identifiers
DOI: 10.1107/S2053230X14024935
PMID: 25484227
Source
Medline
Keywords
License
Unknown

Abstract

Endonuclease III is a bifunctional DNA glycosylase that removes a wide range of oxidized bases in DNA. Deinococcus radiodurans is an extreme radiation-resistant and desiccation-resistant bacterium and possesses three genes encoding endonuclease III enzymes in its genome: DR2438 (EndoIII-1), DR0289 (EndoIII-2) and DR0982 (EndoIII-3). Here, EndoIII-1 and an N-terminally truncated form of EndoIII-3 (EndoIII-3Δ76) have been expressed, purified and crystallized, and preliminary X-ray crystallographic analyses have been performed to 2.15 and 1.31 Å resolution, respectively. The EndoIII-1 crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 181.38, b = 38.56, c = 37.09 Å, β = 89.34° and one molecule per asymmetric unit. The EndoIII-3Δ76 crystals also belonged to the monoclinic space group C2, but with unit-cell parameters a = 91.47, b = 40.53, c = 72.47 Å, β = 102.53° and one molecule per asymmetric unit. The EndoIII-1 structure was determined by molecular replacement, while the truncated EndoIII-3Δ76 structure was determined by single-wavelength anomalous dispersion phasing. Refinement of the structures is in progress.

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