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Expression and promoter analysis of mouse mastrin gene.

Authors
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Volume
307
Issue
3
Pages
491–497
Identifiers
PMID: 12893248
Source
Medline
License
Unknown

Abstract

Human astrin is a newly identified microtubule-associated protein, which is highly expressed in the testis. Silencing of astrin has resulted in growth arrest and apoptotic cell death. In this study, we describe the cloning and genomic structure of mastrin, the mouse counterpart to astrin. The overall mouse mastrin amino-acid sequence is 66% identical to human astrin. Mastrin protein was demonstrated to localize to mitotic spindles during mitosis. Genomic clones containing mastrin gene were isolated; the gene was found to have 24 exons spanning 24kb of genomic DNA. Deletion analysis of 5(')-flanking sequences demonstrated that the first 120bp proximal to the TATA-less promoter region is necessary for minimal transcription of the mouse mastrin gene.

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