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Expression, partial purification and immunogenicity of fragments of the knob protein of Plasmodium falciparum.

Authors
  • Rashid, M A
  • Yang, Y F
  • Kilejian, A
Type
Published Article
Journal
Molecular and Biochemical Parasitology
Publisher
Elsevier
Publication Date
Jan 01, 1990
Volume
38
Issue
1
Pages
49–55
Identifiers
PMID: 2181305
Source
Medline
License
Unknown

Abstract

Three structural domains of the histidine-rich knob protein (KP) of Plasmodium falciparum were expressed in Escherichia coli. A single-step purification scheme was devised to obtain great enrichment of expressed polypeptides for use in subsequent experiments. Immune human sera from Africa, South-East Asia and South America were tested for reactivity with each of the expressed fragments. While the two fragments which represented the central and C-terminal regions of KP showed a strong reactivity with all the antisera which were tested, the N-terminal fragment which contains the repetitive histidine-rich sequences showed almost no reactivity.

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