Expression of the cloned ColE1 kil gene in normal and Kilr Escherichia coli.

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Expression of the cloned ColE1 kil gene in normal and Kilr Escherichia coli.

Publication Date
Nov 01, 1986
Source
PMC
Keywords
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Abstract

The kil gene of the ColE1 plasmid was cloned under control of the lac promoter. Its expression under this promoter gave rise to the same pattern of bacterial cell damage and lethality as that which accompanies induction of the kil gene in the colicin operon by mitomycin C. This confirms that cell damage after induction is solely due to expression of kil and is independent of the cea or imm gene products. Escherichia coli derivatives resistant to the lethal effects of kil gene expression under either the normal or the lac promoter were isolated and found to fall into several classes, some of which were altered in sensitivity to agents that affect the bacterial envelope.

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