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Expression of peptides encoded by exons in cloned mammalian DNA.

Authors
  • S Kreissig
  • K Schüddekopf
  • N Dear
  • T Boehm
Publication Date
Nov 01, 1996
Source
PMC
Keywords
Disciplines
  • Biology
  • Chemistry
  • Mathematics
License
Unknown

Abstract

New synthetic approaches, such as combinatorial chemistry, provide a rich source of potential drug candidates. At the same time, the human genome initiative and other large-scale sequencing projects provide a large number of novel drug targets. However, the functional analysis of thousands of new genes remains a major challenge for the future. A systematic strategy for genome-wide functional analysis of genes could employ the fact that at least some modules in multi-domain proteins are encoded in individual exons. Exon amplification provides information about coding regions of most genes that is independent of their transcriptional status; exon amplification from entire mammalian genomes has been demonstrated. Here, we describe the development of an exon-trap system, lambdaGEE (for genomic exon expression), that couples exon amplification with the expression of exon-encoded peptides.

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