Affordable Access

Access to the full text

Expression of the NADPH+-Dependent Formate-Dehydrogenase Gene from Pseudomonas Increases Lysine Production in Corynebacterium glutamicum

Authors
  • Ryabchenko, L. E.1
  • Leonova, T. E.1
  • Shustikova, T. E.1
  • Gerasimova, T. V.1
  • Ivankova, T. A.1
  • Sidorenko, K. V.1
  • Yanenko, A. S.1
  • 1 State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (Kurchatov Institute NRC, GOSNIIgenetika), Moscow, 117545, Russia , Moscow (Russia)
Type
Published Article
Journal
Applied Biochemistry and Microbiology
Publisher
Pleiades Publishing
Publication Date
Dec 01, 2020
Volume
56
Issue
8
Pages
828–836
Identifiers
DOI: 10.1134/S0003683820080086
Source
Springer Nature
Keywords
License
Yellow

Abstract

AbstractThe psefdh_D221Q gene encodes a mutant formate dehydrogenase from Pseudomonas (P-seFDG_D221Q), which catalyzes formate oxidation with the simultaneous formation of NADPH. It is expressed in cells of lysine-producing Corynebacterium glutamicum strains. The psefdh_D221Q gene was introduced into C. glutamicum strains as part of an autonomous plasmid or was integrated into the chromosome with the simultaneous inactivation of the host formate-dehydrogenase genes. It was shown that the C. glutamicum strains with NADP+-dependent formate dehydrogenase have an increased level of L-lysine synthesis in the presence of formate if their own formate dehydrogenase is inactivated.

Report this publication

Statistics

Seen <100 times