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Expression and long-term stability of a recombinant single-chain Fv antibody fragment in transgenic Nicotiana tabacum seeds

Authors
  • Ramírez, Nadia1
  • Oramas, Pedro1
  • Ayala, Marta2
  • Rodríguez, Meilyn1
  • Pérez, Marlene1
  • Gavilondo, Jorge2
  • 1 Center for Genetic Engineering and Biotechnology, PlantDivision, Havana, Cuba , Havana
  • 2 Center for Genetic Engineering and Biotechnology, Pharmaceuticals Division, Havana, Cuba , Havana
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Jan 01, 2001
Volume
23
Issue
1
Pages
47–49
Identifiers
DOI: 10.1023/A:1026728721966
Source
Springer Nature
Keywords
License
Yellow

Abstract

A functionally active anti-hepatitis B surface antigen single-chain Fv antibody fragment (scFv) was expressed in seeds of transgenic tobacco plants using genetic constructs for expression in the vacuole or the apoplastic fluid. Antibody levels close to 0.2% of the total soluble protein were found. After storage of the transgenic tobacco seeds for one year and a half a year at room temperature, the scFv maintained its antigen-binding activity in full.

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