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[Expression of homologous and heterologous retrotransposons in a model system of yeast cells].

Authors
  • Reznik, N L
  • Gorelova, T V
  • Shuppe, N G
Type
Published Article
Journal
Molecular Genetics Microbiology and Virology (Russian version)
Publisher
Izdatelstvo Meditsina
Publication Date
Jul 01, 1990
Issue
7
Pages
11–17
Identifiers
PMID: 2170833
Source
Medline
License
Unknown

Abstract

Yeast cells Saccharomyces cerevisiae were transformed by the recombinant plasmids containing the Yeast retrotransposon Ty and the Drosophila mobile element gypsy under the control of a strong Yeast promoter. The exogenous Ty-element induces the complete cycle of Ty-retrotransposition including the TyRNA synthesis, formation of virus-like particles, synthesis of all reverse transcriptase intermediates in the virus-like particles with the subsequent circles formation and transposition. The Drosophila mobile element gypsy is capable of inducing the formation of the virus-like particles containing RNA, DNA and proteins of the Ty-retrotransposon only. The Ty-circles and induction of transposition were not observed. The obtained data demonstrates the existence of the multistep repression system for Ty-transposition cycle. The possibility and efficiency of using the model to study the mechanism for retrotransposon transposition is discussed.

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