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Expression of hepatic genes related to energy metabolism during the transition period of Holstein and F1 Holstein-Gir cows.

Authors
  • Laguna, J G1
  • Cardoso, M S2
  • Lima, J A1
  • Reis, R B1
  • Carvalho, A U3
  • Saturnino, H M4
  • Teixeira, S M R5
  • 1 Department of Animal Science, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, 30161-970, Brazil. , (Brazil)
  • 2 Department of Parasitology, UFMG, Belo Horizonte, 31270-901, Brazil. , (Brazil)
  • 3 Department of Clinical and Surgery, UFMG, Belo Horizonte, 30161-970, Brazil. , (Brazil)
  • 4 Department of Animal Science, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, 30161-970, Brazil. Electronic address: [email protected] , (Brazil)
  • 5 Biochemistry and Immunology Department, UFMG, Belo Horizonte, 31270-901, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Journal of Dairy Science
Publisher
American Dairy Science Association
Publication Date
Dec 01, 2017
Volume
100
Issue
12
Pages
9861–9870
Identifiers
DOI: 10.3168/jds.2016-12459
PMID: 28964523
Source
Medline
Keywords
License
Unknown

Abstract

The aim of this study was to investigate the expression of genes encoding enzymes and other factors involved with carbohydrate and lipid metabolism in the liver of 2 genetic groups of dairy cows during the transition period. We analyzed the expression of glucose-6-phosphatase (G6PC), cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), methylmalonyl-CoA mutase (MUT), β-hydroxybutyrate dehydrogenase-2 (BDH2), acetyl-CoA carboxylase (ACC), carnitine palmitoyltransferase-2 (CPT2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), glucose transporter-2 (SLC2A2), and the transcription factor peroxisome proliferator-activated receptor α (PPARA). Blood concentrations of glucose, nonesterified fatty acids, and β-hydroxybutyrate were also determined. Liver biopsies and blood samples were taken at d 15 prepartum and at d 6, 21, 36, 51, and 66 postpartum from Holsteins (n = 6) and F1 Holstein-Gir (n = 6) cows. Cows were kept under the same prepartum and postpartum management conditions. The results showed that the expression of G6PC, PEPCK-C, BDH2, ACC, CPT2, HMGCR, SLC2A2, and PPARA genes did not differ between genetic groups. Except for PEPCK-C, no interaction between genetic groups and the experimental period was observed. Within both groups of cows, G6PC and PEPCK-C gene expression decreased when comparing prepartum gene expression with 21 and 36 DIM, and increased in d 51 postpartum. MUT mRNA levels differed between the 2 genetic groups and displayed a significant increase after d 36 postpartum, whereas mRNA levels of HMGCR tended to increase when comparing d 21 and 36 to d 51 postpartum. Glucose concentrations also differed between genetic groups, being significantly higher in the plasma of F1 Holstein-Gir cows than in Holstein cows, but no differences were found within each group during the analysis period. β-Hydroxybutyrate and nonesterified fatty acid concentrations did not differ between genetic groups, but displayed increased levels from prepartum to d 6 and 21 postpartum. Our results indicated that expression in the liver of genes involved with glucose and fatty acid metabolism were similar in both groups of cows and significant differences were observed between the 2 groups in the expression of MUT, a gene involved in propionate metabolism.

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