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Expression of Genomic Functional Estrogen Receptor 1 in Mouse Sertoli Cells

Authors
  • Lin, Jing1
  • Zhu, Jia1
  • Li, Xian1
  • Li, Shengqiang1
  • Lan, Zijian2
  • Ko, Jay3
  • Lei, Zhenmin1
  • 1 University of Louisville School of Medicine, Louisville, KY, USA , Louisville (United States)
  • 2 Alltech, Nicholasville, KY, USA , Nicholasville (United States)
  • 3 University of Illinois at Urbana-Champaign, Urbana, IL, USA , Urbana (United States)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Nov 01, 2014
Volume
21
Issue
11
Pages
1411–1422
Identifiers
DOI: 10.1177/1933719114527355
Source
Springer Nature
Keywords
License
Yellow

Abstract

There is no consensus whether Sertoli cells express estrogen receptor 1 (Esr1). Reverse transcription-polymerase chain reaction, Western blot, and immunofluorescence demonstrated that mouse Sertoli cell lines, TM4, MSC-1, and 15P-1, and purified primary mouse Sertoli cells (PSCs) contained Esr1 messenger RNA and proteins. Incubation of Sertoli cells with 17β-estradiol (E2) or ESR1 agonist stimulated the expression of an estrogen responsive gene Greb1, which was prevented by ESR inhibitor or ESR1 antagonist. Overexpression of Esr1 in MSC-1 enhanced E2-induced Greb1 expression, while knockdown of Esr1 by small interfering RNA in TM4 attenuated the response. Furthermore, E2-induced Greb1 expression was abolished in the PSCs isolated from Amh-Cre/Esr1-floxed mice in which Esr1 in Sertoli cells were selectively deleted. Chromatin immunoprecipitation assays indicated that E2-induced Greb1 expression in Sertoli cells was mediated by binding of ESR1 to estrogen responsive elements. In summary, ligand-dependent nuclear ESR1 was present in mouse Sertoli cells and mediates a classical genomic action of estrogens.

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