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Expression of the gene coding for the major outer capsid protein of SA-11 rotavirus in a baculovirus system.

Authors
  • McGonigal, T P
  • Turon, M C
  • Komar-Hartnett, K
  • Kister, S E
  • Smith, R E
Type
Published Article
Journal
Virus Research
Publisher
Elsevier
Publication Date
Apr 01, 1992
Volume
23
Issue
1-2
Pages
135–150
Identifiers
PMID: 1318623
Source
Medline
License
Unknown

Abstract

The gene coding for the major outer capsid protein (VP7) of simian rotavirus SA-11 has been expressed in a baculovirus-insect cell system. The resulting protein is 35 kDa and is primarily associated with the endoplasmic reticulum. Neutralizing SA-11 polyclonal antiserum and VP7 monospecific antiserum reacted specifically with the expressed gene product. Antiserum derived against the recombinant VP7 protein neutralized SA-11 rotavirus infectivity in a fluorescent focus assay.

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