Expression of the gene coding for the major outer capsid protein of SA-11 rotavirus in a baculovirus system.
- Published Article
- Publication Date
Apr 01, 1992
The gene coding for the major outer capsid protein (VP7) of simian rotavirus SA-11 has been expressed in a baculovirus-insect cell system. The resulting protein is 35 kDa and is primarily associated with the endoplasmic reticulum. Neutralizing SA-11 polyclonal antiserum and VP7 monospecific antiserum reacted specifically with the expressed gene product. Antiserum derived against the recombinant VP7 protein neutralized SA-11 rotavirus infectivity in a fluorescent focus assay.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/1318623