Affordable Access

Expression of foreign DNA in Chlamydomonas reinhardtii.

Authors
Type
Published Article
Journal
FEMS microbiology letters
Publication Date
Volume
53
Issue
1-2
Pages
77–81
Identifiers
PMID: 2558951
Source
Medline

Abstract

A chimeric octopine synthase-neomycin phosphotransferase (ocs-nptII) gene was used to transform Chlamydomonas reinhardiii to kanamycin resistance. Southern hybridization using DNA isolated from one transformant, T6.1, indicated that the entire ocs-nptII gene and at least part of the plasmid were integrated into nuclear DNA. Neomycin phosphotransferase II activity has been detected in T6.1 cell extracts. Northern hybridizations, employing a radiolabeled ocs-nptII sequence, revealed a T6.1 transcript of approximately the same size as a homologous transcript isolated from E. coli carrying the nptII gene. Although T6.1 is an extremely rare example of a stable C. reinhardtii transformant, its occurrence nevertheless indicates that bacterial genes can be expressed in the nucleus of the alga.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments