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Expression of foreign DNA in Chlamydomonas reinhardtii.

Authors
  • Bingham, S E
  • Cox, J C
  • Strem, M D
Type
Published Article
Journal
FEMS microbiology letters
Publication Date
Nov 01, 1989
Volume
53
Issue
1-2
Pages
77–81
Identifiers
PMID: 2558951
Source
Medline
License
Unknown

Abstract

A chimeric octopine synthase-neomycin phosphotransferase (ocs-nptII) gene was used to transform Chlamydomonas reinhardiii to kanamycin resistance. Southern hybridization using DNA isolated from one transformant, T6.1, indicated that the entire ocs-nptII gene and at least part of the plasmid were integrated into nuclear DNA. Neomycin phosphotransferase II activity has been detected in T6.1 cell extracts. Northern hybridizations, employing a radiolabeled ocs-nptII sequence, revealed a T6.1 transcript of approximately the same size as a homologous transcript isolated from E. coli carrying the nptII gene. Although T6.1 is an extremely rare example of a stable C. reinhardtii transformant, its occurrence nevertheless indicates that bacterial genes can be expressed in the nucleus of the alga.

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