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Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources.

Authors
  • Yuan, Bo
  • Wang, Shi-An
  • Li, Fu-Li
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Oct 01, 2013
Volume
35
Issue
10
Pages
1589–1592
Identifiers
DOI: 10.1007/s10529-013-1251-1
PMID: 23743955
Source
Medline
License
Unknown

Abstract

To improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast Kluyveromyces marxianus and the recently identified yeast, Candida kutaonensis, were expressed in Saccharomyces cerevisiae. S. cerevisiae harboring the exoinulinase gene from C. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l(-1) h(-1)) and Jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l(-1) h(-1)) compared with the strain expressing the exoinulinase gene from K. marxianus. Thus, the exoinulinase gene from C. kutaonensis is advantageous for engineering S. cerevisiae to improve ethanol fermentation from inulin sources.

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