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Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase.

Authors
  • Gatenby, A A
  • Castleton, J A
  • Saul, M W
Type
Published Article
Journal
Nature
Publication Date
May 14, 1981
Volume
291
Issue
5811
Pages
117–121
Identifiers
PMID: 7015143
Source
Medline
License
Unknown

Abstract

Synthesis of the large subunit polypeptide of ribulose bisphosphate carboxylase can be detected in Escherichia coli cells containing the chloroplast genes from maize and wheat. The chloroplast DNA sequences contain a 'promoter' that, in Escherichia coli, initiates transcription and translation of the genes. This synthesis of large subunits has been substantially increased using the powerful lambda promoter, PL, to drive transcription of the maize gene.

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