Affordable Access

deepdyve-link
Publisher Website

Expression dynamics of autophagy-related genes in the cattle tick Rhipicephalus microplus during embryonic development and under increasing larval starvation.

Authors
  • González Castillo, Celia1, 2
  • Ortuño Sahagún, Daniel1
  • Martínez Velázquez, Moisés3
  • 1 Instituto de Investigación en Ciencias Biomédicas (IICB), CUCS, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, 44340, Guadalajara, Jalisco, Mexico. , (Mexico)
  • 2 Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, Zapopan, Mexico. , (Mexico)
  • 3 Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico. [email protected] , (Mali)
Type
Published Article
Journal
Experimental and Applied Acarology
Publisher
Springer-Verlag
Publication Date
Oct 01, 2019
Volume
79
Issue
2
Pages
255–266
Identifiers
DOI: 10.1007/s10493-019-00428-7
PMID: 31659545
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Rhipicephalus microplus is a hematophagous ectoparasite that significantly affects parasitized cattle. As a one-host tick its entire life cycle consists of free-living and parasitic forms. Its extraordinary ability to survive during prolonged off-host periods has been related to the process of cytoplasmic degradation called autophagy. In order to deepen our understanding of this process during R. microplus non-parasitic stages, we determined the expression dynamics of a set of five autophagy-related genes (ATG genes) during embryonic development and over an increasing larval starvation period of 50 days. We found two apparent successive waves of ATG genes transcriptional activation, which paralleled key embryonic changes such as cellularization and organogenesis, as well as nutrient utilization. Moreover, during increasing larval starvation, ATG genes were up-regulated cyclically every 10-15 days. Taken together, our results suggest that autophagy is playing a major role in embryo development and energy metabolism during starvation in R. microplus.

Report this publication

Statistics

Seen <100 times