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Expression of deubiquitinases in human gingiva and cultured human gingival fibroblasts

Authors
  • Fu, Yong-Wei1
  • Xu, Hong-Zhi1
  • 1 The First People’s Hospital of Lianyungang (Lianyungang Clinical Medical College of Nanjing Medical University), 6 East Zhenhua Road, Haizhou District, Lianyungang, Jiangsu, 222061, China , Lianyungang (China)
Type
Published Article
Journal
BMC Oral Health
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jun 06, 2021
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12903-021-01655-4
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundAlthough deubiquitinating enzymes (DUBs) such as CYLD, A20 and OTULIN are expressed in multiple tissues and thought to be linked with inflammatory diseases, their expression in periodontal tissues remains to be determined. This research was designed to assess the expression of CYLD, A20 and OTULIN in human gingiva, and to evaluate the regulation of these DUBs in human gingival fibroblasts (HGFs) upon different stimuli.MethodsImmunohistochemistry assay was conducted to determine the expression of CYLD, A20 and OTULIN in human gingiva. Immunofluorescence assay was employed to observe the protein expression of CYLD, A20 and OTULIN in HGFs. RT-PCR and western blots were carried out to assess gene and protein expression changes of these DUBs in HGFs upon LPS or TNF-α.ResultsCYLD, A20 and OTULIN were found to be expressed in human gingiva and HGFs. The expression of CYLD, A20 and OTULIN was lower in the inflamed gingival tissue samples compared with the healthy gingival tissue samples. Further, the expression of CYLD, A20 and OTULIN in HGFs exhibited distinct regulation by different stimuli. TNF-α treatment markedly increased NF-κB activation in HGFsConclusionsOur findings suggest that CYLD, A20 and OTULIN might play a role in the progression of periodontitis.

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