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Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68.

Authors
  • Samadlouie, Hamid-Reza
  • Hamidi-Esfahani, Zohreh
  • Alavi, Seyed-Mehdi
  • Varastegani, Boshra
Type
Published Article
Journal
Brazilian Journal of Microbiology
Publisher
Elsevier
Publication Date
Jan 01, 2014
Volume
45
Issue
2
Pages
439–445
Identifiers
PMID: 25242926
Source
Medline
Keywords
License
Unknown

Abstract

The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.

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