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[Experimental study on antitumor effect in vitro of modified DNL of oral squamous cell carcinoma with TNF-alpha gene].

Authors
Type
Published Article
Journal
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
Publication Date
Volume
32
Issue
5
Pages
262–264
Identifiers
PMID: 11189281
Source
Medline
License
Unknown

Abstract

The gene coding for TNF-alpha was introduced into DNL of human oral squamous carcinoma by retroviral-mediated gene transduction, and experiments proved that the gene had been introduced into the DNL and the DNL could secrete TNF-alpha of high activity with PCR analysis, bioactivity analysis of TNF-alpha, selection test in G418. Cytotoxicity of gene-transduced and nontransduced DNL to Tca8113 cell was compared with MTT method. The result showed that cytotoxicity of the former was higher than that of the latter.

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