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Experimental silicosis does not aggravate collagen-induced arthritis in mice

Authors
  • Engelmann, Robby1
  • Müller-Hilke, Brigitte1
  • 1 Rostock University Medical Center, Institute of Immunology & Core Facility for Cell Sorting & Cell Analysis, Schillingallee 70, Rostock, 18057, Germany , Rostock (Germany)
Type
Published Article
Journal
Journal of Negative Results in BioMedicine
Publisher
BioMed Central
Publication Date
Mar 13, 2017
Volume
16
Issue
1
Identifiers
DOI: 10.1186/s12952-017-0071-6
Source
Springer Nature
Keywords
License
Green

Abstract

ObjectivesTo investigate the effect of chronic lung inflammation on the incidence and severity of collagen-induced arthritis in mice.MethodsChronic lung inflammation in the form of silicosis was induced via intranasal application of silica particles. Immunization with collagen Type II commenced one week later and mice were sacrificed six weeks after booster immunization. Thereafter, silicosis was confirmed via flow cytometry and arthritis was evaluated performing knee and paw histology.ResultsPronounced lung inflammation in the silica-treated compared to PBS-treated control mice was demonstrated by significantly elevated broncho-alveolar lavage (BAL) cell count, attributable to increased numbers of macrophages and granulocytes. Inflammation in the lungs was not associated with elevated PAD2 and PAD4 expression, yet silica treated animals had significantly higher aCCP serum titers. However, lung inflammation did not lead to an increase in the incidence of arthritis, nor did it exacerbate the macroscopic or histologic joint scores.ConclusionsChronic lung inflammation resulting from silicosis does not aggravate collagen-induced arthritis in mice.

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