Affordable Access

deepdyve-link
Publisher Website

Exosome secretion is enhanced by invadopodia and drives invasive behavior.

Authors
  • Hoshino, Daisuke
  • Kirkbride, Kellye C
  • Costello, Kaitlin
  • Clark, Emily S
  • Sinha, Seema
  • Grega-Larson, Nathan
  • Tyska, Matthew J
  • Weaver, Alissa M
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Dec 12, 2013
Volume
5
Issue
5
Pages
1159–1168
Identifiers
DOI: 10.1016/j.celrep.2013.10.050
PMID: 24290760
Source
Medline
License
Unknown

Abstract

Unconventional secretion of exosome vesicles from multivesicular endosomes (MVEs) occurs across a broad set of systems and is reported to be upregulated in cancer, where it promotes aggressive behavior. However, regulatory control of exosome secretion is poorly understood. Using cancer cells, we identified specialized invasive actin structures called invadopodia as specific and critical docking and secretion sites for CD63- and Rab27a-positive MVEs. Thus, inhibition of invadopodia formation greatly reduced exosome secretion into conditioned media. Functionally, addition of purified exosomes or inhibition of exosome biogenesis or secretion greatly affected multiple invadopodia life cycle steps, including invadopodia formation, stabilization, and exocytosis of proteinases, indicating a key role for exosome cargoes in promoting invasive activity and providing in situ signaling feedback. Exosome secretion also controlled cellular invasion through three-dimensional matrix. These data identify a synergistic interaction between invadopodia biogenesis and exosome secretion and reveal a fundamental role for exosomes in promoting cancer cell invasiveness.

Report this publication

Statistics

Seen <100 times