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Exogenous melatonin alleviates cadmium uptake and toxicity in apple rootstocks.

Authors
  • He, Jiali1, 2
  • Zhuang, Xiaolei1, 2
  • Zhou, Jiangtao1, 3
  • Sun, Luyang1, 2
  • Wan, Huixue1, 2
  • Li, Huifeng4
  • Lyu, Deguo1, 2
  • 1 College of Horticulture, Shenyang Agricultural University, 120 Dongling Rd, Shenhe District, Shenyang, Liaoning 110866, People's Republic of China. , (China)
  • 2 Key Lab of Fruit Quality Development and Regulation of Liaoning Province, Shenyang Agricultural University, 120 Dongling Rd, Shenhe District, Shenyang, Liaoning, 110866, People's Republic of China. , (China)
  • 3 Research Institute of Pomology, Chinese Academy of Agricultural Sciences Xingcheng, 98 Xinghai South Street, Liaoning 125100, People's Republic of China. , (China)
  • 4 Institute of Pomology, Shandong Academy of Agricultural Sciences, Tailai Rd, Taishan, Tai'an 271000, People's Republic of China. , (China)
Type
Published Article
Journal
Tree Physiology
Publisher
Oxford University Press
Publication Date
May 30, 2020
Volume
40
Issue
6
Pages
746–761
Identifiers
DOI: 10.1093/treephys/tpaa024
PMID: 32159805
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

To examine the potential roles of melatonin in cadmium (Cd) uptake, accumulation and detoxification in Malus plants, we exposed two different apple rootstocks varying greatly in Cd uptake and accumulation to either 0 or 30 μM Cd together with 0 or 100 μM melatonin. Cadmium stress stimulated endogenous melatonin production to a greater extent in the Cd-tolerant Malus baccata Borkh. than in the Cd-susceptible Malus micromalus 'qingzhoulinqin'. Melatonin application attenuated Cd-induced reductions in growth, photosynthesis and enzyme activity, as well as reactive oxygen species (ROS) and malondialdehyde accumulation. Melatonin treatment more effectively restored photosynthesis, photosynthetic pigments and biomass in Cd-challenged M. micromalus 'qingzhoulinqin' than in Cd-stressed M. baccata. Exogenous melatonin lowered root Cd2+ uptake, reduced leaf Cd accumulation, decreased Cd translocation factors and increased root, stem and leaf melatonin contents in both Cd-exposed rootstocks. Melatonin application increased both antioxidant concentrations and enzyme activities to scavenge Cd-induced ROS. Exogenous melatonin treatment altered the mRNA levels of several genes regulating Cd uptake, transport and detoxification including HA7, NRAMP1, NRAMP3, HMA4, PCR2, NAS1, MT2, ABCC1 and MHX. Taken together, these results suggest that exogenous melatonin reduced aerial parts Cd accumulation and mitigated Cd toxicity in Malus plants, probably due to the melatonin-mediated Cd allocation in tissues, and induction of antioxidant defense system and transcriptionally regulated key genes involved in detoxification. © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: [email protected]

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