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Exercise training delays cardiac remodeling in a mouse model of cancer cachexia.

Authors
  • Fernandes, L G1
  • Tobias, G C2
  • Paixão, A O2
  • Dourado, P M3
  • Voltarelli, V A2
  • Brum, P C4
  • 1 Department of Experimental Pathophysiology, Medical School, University of Sao Paulo, Sao Paulo, Brazil; School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil. , (Brazil)
  • 2 School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil. , (Brazil)
  • 3 Heart Institute, Clinical Hospital, Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil. , (Brazil)
  • 4 School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Life sciences
Publication Date
Sep 06, 2020
Volume
260
Pages
118392–118392
Identifiers
DOI: 10.1016/j.lfs.2020.118392
PMID: 32898523
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We aimed to investigate the impact of cancer cachexia and previous aerobic exercise training (AET) on cardiac function and structure in tumor bearing mice. Colon adenocarcinoma cells 26 (CT26) were subcutaneously injected in BALB/c mice to establish robust cancer cachexia model. AET was performed on a treadmill during 45 days, 60 min/5 days per week. Cardiac function was evaluated by echocardiography and cardiac morphology was assessed by light microscopy. The protein expression levels of mitochondrial complex were analyzed by Western blotting. The mRNA levels of genes related to cardiac remodeling and autophagy were analyzed by quantitative Real-Time PCR. Our data confirms CT26 tumor bearing mice as a well-characterized and robust model of cancer cachexia. CT26 mice exhibited cardiac remodeling and dysfunction characterized by cardiac atrophy and impaired left ventricle ejection fraction paralleled by cardiac necrosis, inflammation and fibrosis. AET partially reversed the left ventricle ejection fraction and led to significant anti-cardiac remodeling effect associated reduced necrosis, inflammation and cardiac collagen deposition in CT26 mice. Reduced TGF-β1 mRNA levels, increased mitochondrial complex IV protein levels and partial recovery of BNIP3 mRNA levels in cardiac tissue were associated with the cardiac effects of AET in CT26 mice. Thus, we suggest AET as a powerful regulator of key pathways involved in cardiac tissue homeostasis in cancer cachexia. Our study provides a robust model of cancer cachexia, as well as highlights the potential and integrative effects of AET as a preventive strategy for reducing cardiac damage in cancer cachexia. Copyright © 2020 Elsevier Inc. All rights reserved.

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