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[The ex vivo expansion of cord blood hematopoietic cells: 1. Cell growth behavior].

Authors
  • Zhang, Y X1
  • Ying, X F
  • Tan, W S
  • 1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237.
Type
Published Article
Journal
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Publication Date
September 2000
Volume
16
Issue
5
Pages
623–626
Identifiers
PMID: 11191771
Source
Medline
License
Unknown

Abstract

The population growth and metabolism of hematopoietic cells from cord blood were investigated without and with hematopoietic growth factors and the long-term growth kinetics of cells was studied. In static cultivation of hematopoietic cells, the population specific growth rate was 0.34 d-1, and the average double time 2 days. The greater part of glucose was consumed and 40 mmol/L lactate was formed during cell culture. For the long-term culture, the maximum output of CFU-GM appeared between the 2nd and 3rd weeks and that of BFU-E in the first week. With 50% medium exchange, the expansions of total cells, CFU-GM, and BFU-E came up to 14, 13, and 5 times, respectively.

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