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Evolutionary history of double-stranded RNA binding proteins in plants: identification of new cofactors involved in easiRNA biogenesis

Authors
  • Clavel, Marion1, 2
  • Pélissier, Thierry3
  • Montavon, Thomas4
  • Tschopp, Marie-Aude5
  • Pouch-Pélissier, Marie-Noëlle3
  • Descombin, Julie1, 2
  • Jean, Viviane1, 2
  • Dunoyer, Patrice4
  • Bousquet-Antonelli, Cécile1, 2
  • Deragon, Jean-Marc1, 2
  • 1 Université de Perpignan Via Domitia, UMR5096 LGDP, 58 Avenue Paul Alduy, Perpignan Cedex, 66860, France , Perpignan Cedex (France)
  • 2 CNRS UMR5096 LGDP, Perpignan Cedex, France , Perpignan Cedex (France)
  • 3 Clermont Université, UMR 6293 CNRS - INSERM U1103 – GreD, 24 avenue des Landais, Aubière Cedex, 63171, France , Aubière Cedex (France)
  • 4 Université de Strasbourg, Institut de Biologie Moléculaire des Plantes du CNRS, UPR2357, Strasbourg Cedex, France , Strasbourg Cedex (France)
  • 5 ETH Zürich, Department of Biology LFW D17/D18, Universitätsstrasse 2, Zurich, 8092, Switzerland , Zurich (Switzerland)
Type
Published Article
Journal
Plant Molecular Biology
Publisher
Springer Netherlands
Publication Date
Feb 09, 2016
Volume
91
Issue
1-2
Pages
131–147
Identifiers
DOI: 10.1007/s11103-016-0448-9
Source
Springer Nature
Keywords
License
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Abstract

In this work, we retrace the evolutionary history of plant double-stranded RNA binding proteins (DRBs), a group of non-catalytic factors containing one or more double-stranded RNA binding motif (dsRBM) that play important roles in small RNA biogenesis and functions. Using a phylogenetic approach, we show that multiple dsRBM DRBs are systematically composed of two different types of dsRBMs evolving under different constraints and likely fulfilling complementary functions. In vascular plants, four distinct clades of multiple dsRBM DRBs are always present with the exception of Brassicaceae species, that do not possess member of the newly identified clade we named DRB6. We also identified a second new and highly conserved DRB family (we named DRB7) whose members possess a single dsRBM that shows concerted evolution with the most C-terminal dsRBM domain of the Dicer-like 4 (DCL4) proteins. Using a BiFC approach, we observed that Arabidopsis thaliana DRB7.2 (AtDRB7.2) can directly interact with AtDRB4 but not with AtDCL4 and we provide evidence that both AtDRB7.2 and AtDRB4 participate in the epigenetically activated siRNAs pathway.

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