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Evidence for the subsynaptic zone as a preferential site for CHRN recycling at neuromuscular junctions.

Authors
  • Wild, Franziska1, 2, 3
  • Khan, Muzamil Majid1, 2, 3
  • Rudolf, Rüdiger1, 2, 3
  • 1 a Institute of Molecular and Cell Biology, Mannheim University of Applied Sciences , Mannheim , Germany. , (Germany)
  • 2 b Interdisciplinary Center for Neurosciences, Heidelberg University , Heidelberg , Germany. , (Germany)
  • 3 c Institute of Toxicology and Genetics, Karlsruhe Institute of Technology , Eggenstein-Leopoldshafen , Germany. , (Germany)
Type
Published Article
Journal
Small GTPases
Publication Date
Sep 01, 2019
Volume
10
Issue
5
Pages
395–402
Identifiers
DOI: 10.1080/21541248.2017.1324939
PMID: 28489965
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Vertebrate skeletal muscle contraction is mediated by nicotinic acetylcholine receptors (CHRN). Endocytosis and recycling of CHRN regulate their proper abundance at nerve-muscle synapses, i.e. neuromuscular junctions. Recent work showed that RAB5 is essential for CHRN endocytosis. Here, using in vivo-imaging of endocytosed CHRN and RAB-GFP fusion proteins, we deliver evidence for differential effects of RAB5-GFP, RAB4-GFP, and RAB11-GFP on CHRN endocytosis. Furthermore, while newly endocytosed CHRN colocalized with RAB5-GFP over large stretches of muscle fibers, RAB4-GFP and RAB11-GFP colocalized with endocytosed CHRN almost exclusively at neuromuscular junctions. In agreement with previous findings, this data suggests the existence of a specialized subsynaptic zone that is particularly relevant for CHRN recycling.

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