Affordable Access

Evidence for a recent mutation giving rise to a truncated copy of a cysteine proteinase gene in Leishmania pifanoi.

Authors
  • Boukai, L K
  • McMahon-Pratt, D
  • Traub-Cseko, Y M
Type
Published Article
Journal
Parasitology international
Publication Date
Dec 01, 2000
Volume
49
Issue
4
Pages
301–307
Identifiers
PMID: 11077264
Source
Medline
License
Unknown

Abstract

We have previously identified and characterized two amastigote-specific cysteine proteinases of Leishmania pifanoi. The slightly different isoforms of the more abundant proteinase are coded by a gene family of approximately 20 gene copies, that contain a C-terminal extension characteristic of cysteine proteinases of trypanosomatids. In this gene family, we have detected a copy that codes for a truncated form of this proteinase, lacking the C-terminal extension. Interestingly, when the deletion of a nucleotide that creates a stop codon causing this truncation is disregarded, the translated sequence gives rise to a divergent C-terminal extension that has many conserved amino acids when compared to Leishmania and Trypanosome, suggesting that a recent mutation led to the truncation.

Report this publication

Statistics

Seen <100 times