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Evidence for non-chromosomal hepatitis B virus surface (HBsAg)- and core antigen (HBcAg)-specific DNA sequences in a hepatoma cell line.

Authors
  • Marquardt, O
  • Zaslavsky, V
  • Hofschneider, P H
Type
Published Article
Journal
The Journal of general virology
Publication Date
Jul 01, 1982
Volume
61 (Pt l)
Pages
105–109
Identifiers
PMID: 6288845
Source
Medline
License
Unknown

Abstract

As demonstrated previously, a "beta particle" fraction isolated from the cytoplasm of PLC/PRF/5 cells contains hepatitis B virus (HBV)-specific DNA. Here, further evidence is provided that the specificity of the DNA for HBV is represented at least by sequences coding for the surface and core antigen (HBsAg and HBcAg). This was shown by two different hybridization techniques. One of them, the technique of Southern, distinguished these hybrid molecules formed from those containing HBV DNA integrated into chromosomes. The HBV-specific beta particle DNA forms two distinct bands separate from the high molecular weight cellular DNA.

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