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Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus.

Authors
Type
Published Article
Journal
PLANT PHYSIOLOGY
0032-0889
Publisher
American Society of Plant Biologists
Publication Date
Volume
97
Issue
2
Pages
829–831
Identifiers
PMID: 16668476
Source
Medline

Abstract

Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.

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