Affordable Access

Evidence for a localization signal in the 3'untranslated region of myosin heavy chain messenger RNA.

Authors
  • Wiseman, J W
  • Glover, L A
  • Hesketh, J E
Type
Published Article
Journal
Cell biology international
Publication Date
Apr 01, 1997
Volume
21
Issue
4
Pages
243–248
Identifiers
PMID: 9204538
Source
Medline
License
Unknown

Abstract

Localization signals in the 3'untranslated region (3'UTR) of myosin heavy chain mRNA were investigated using hybrid gene constructs. In myoblasts transfected with constructs containing either both coding sequences and 3'UTR of the rabbit beta-globin gene or the beta-globin coding sequences alone in situ hybridization showed that globin transcripts were distributed throughout the cytoplasm with no localization. In contrast, in myoblasts transfected with beta-globin coding sequences linked to the myosin heavy chain 3'UTR there was strong perinuclear localization of the hybrid mRNA; this was maintained in myotubes. We conclude that myosin heavy chain 3'UTR contains a localization signal.

Report this publication

Statistics

Seen <100 times