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Evidence for energy-dependent transposition of core lipopolysaccharide across the inner membrane of Salmonella typhimurium.

Authors
Type
Published Article
Journal
Journal of Bacteriology
0021-9193
Publisher
American Society for Microbiology
Publication Date
Volume
173
Issue
10
Pages
3134–3137
Identifiers
PMID: 1708762
Source
Medline

Abstract

The uncoupler 2,4-dinitrophenol blocks the final step of lipopolysaccharide assembly--transfer of O antigen from undecaprenyl pyrophosphate to core lipopolysaccharide--in intact Salmonella typhimurium but not in isolated membrane fractions. The O-antigen ligase enzyme is not inhibited by dinitrophenol in vitro, and core lipopolysaccharide synthesized in the presence of uncoupler in vivo is functional as acceptor of O antigen in vitro. The evidence strongly suggests that maintenance of proton motive force is required for transmembrane transposition of core lipopolysaccharide to the active site of O-antigen ligase at the periplasmic face of the inner membrane.

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