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Evidence for age-dependent impairment of ovalbumin heterogeneous nuclear RNA (HnRNA) processing in hen oviduct.

Authors
  • Schröder, H C
  • Messer, R
  • Breter, H J
  • Müller, W E
Type
Published Article
Journal
Mechanisms of Ageing and Development
Publisher
Elsevier
Publication Date
May 31, 1985
Volume
30
Issue
3
Pages
319–324
Identifiers
PMID: 4021562
Source
Medline
License
Unknown

Abstract

The expression of the ovalbumin gene in hen oviduct decreases during ageing. Northern transfer of electrophoretically fractionated total RNA from oviducts of mature egg-laying and old non-egg-laying hens, and hybridization to nick-translated plasmid pOV230 (ovalbumin complementary DNA cloned into pMB9) revealed with age increasing concentrations of distinct high-molecular-weight ovalbumin pre-mRNA species as compared with that of functional 18S ovalbumin mRNA. The accumulation of ovalbumin sequence-containing processing intermediates in old hen oviducts indicates lowered rates of RNA splicing and/or altered steady state concentrations of splicing intermediates during post-transcriptional maturation of heterogeneous nuclear RNA in old animals.

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