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Evaluation of VIASURE Sexually Transmitted Diseases Real Time PCR Detection kit (CerTest Biotec) for the diagnostic of sexually transmitted infections.

Authors
  • Casañ López, Cristina1
  • Rivaya Sánchez, Belén2
  • Fernández Rivas, Gema3
  • Hernández Rodríguez, Águeda3
  • Antuori Torres, Adrián2
  • Linares Llamas, Georgina2
  • Matas Andreu, Lurdes4
  • 1 Servicio de Microbiología, Laboratorio Clínico Metropolitana Norte, Hospital Universitari Germans Trias i Pujol, Badalona, Spain. Electronic address: [email protected] , (Spain)
  • 2 Servicio de Microbiología, Laboratorio Clínico Metropolitana Norte, Hospital Universitari Germans Trias i Pujol, Badalona, Spain. , (Spain)
  • 3 Servicio de Microbiología, Laboratorio Clínico Metropolitana Norte, Hospital Universitari Germans Trias i Pujol, Badalona, Spain; Departamento de Genética y Microbiología, Universidad Autónoma de Barcelona, Badalona, Spain. , (Spain)
  • 4 Servicio de Microbiología, Laboratorio Clínico Metropolitana Norte, Hospital Universitari Germans Trias i Pujol, Badalona, Spain; Departamento de Genética y Microbiología, Universidad Autónoma de Barcelona, Badalona, Spain; CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. , (Spain)
Type
Published Article
Journal
Enfermedades infecciosas y microbiologia clinica (English ed.)
Publication Date
May 01, 2021
Volume
39
Issue
5
Pages
229–233
Identifiers
DOI: 10.1016/j.eimc.2020.04.013
PMID: 32498967
Source
Medline
Keywords
Language
Spanish
License
Unknown

Abstract

Sexually transmitted infections (STIs) are common in our environment, and trends have been increasing in the last few years. Different methods for STIs diagnosis have been applied by microbiology laboratories over years, but real-time PCR has improved this process. Our objective was to evaluate VIASURE Sexually Transmitted Diseases Real-Time PCR Detection kit (CerTestBiotec, S.L.) comparing with the real-time PCR technique used in our laboratory (Allplex™ STI7 Essential Assay, Seegene) which was considered as reference assay. A total of 948 samples from different sites (vaginal, endocervical, urethral, rectal, pharyngeal swabs and urine samples) were analyzed from July to September 2018. A discordant result was obtained in 4.5% (43 samples). These discrepancies were mainly observed in threshold cycle (Ct) value next to the limit of detection. The k coefficient obtained shows a very high agreement between both methods with k values from 0.92 to 0.99. VIASURE Sexually Transmitted Diseases Real-Time PCR Detection kit provides a very good correlation with Allplex STI7 and therefore, it's a good tool for the diagnostic of STIs. Positive results with Ct value obtained from 35 and low amplification signal should be applied with caution and should be interpreted based on the patient's clinical data. Copyright © 2020 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

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